Thurs. Dec. 6th - Mon Dec. 10th

Transformation Lab!!!

In this experiment we will be creating a transgenic organism in our lab.  We will be taking the genes from glowing organisms (jellyfish and coral) and putting them into bacteria to make GLOWING BACTERIA!

Agenda:
Thurs: Background Info (Pre-lab done in class or finished at lunch)
Fri: Lab Day (yay!)
Mon: results

Pre Lab: YOU CANNOT DO THE LAB WITHOUT A FINISHED PRE-LAB.  NO EXCEPTIONS.
Title: Bacterial Transformation
Objective: To perform and observe the process of bacterial transformation
Procedure: (copy from below)
Pre-lab questions:
1.) What does the Ampicillin do? How do we know what plate has ampicillin on it?
2.)  What genes or on the plasmid?
3.)  What does the "+" and the "-" mean on the tubes?